Composite
Part:BBa_K4164023:Design
Designed by: Bo Cheng Group: iGEM22_NAU-CHINA (2022-09-30)
J23105-FXR-Cadc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 117
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1700
Design Notes
We recombined low intensity promoter (BBa_J23105) and RBS (BBa_B0034) and FXR-CadC (BBa_K4164026) by homologous recombination.
Source
This sequence is composed of BBa_J23105, BBa_B0034 and BBa_K4164027.