Composite

Part:BBa_K4164023:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


J23105-FXR-Cadc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1700


Design Notes

We recombined low intensity promoter (BBa_J23105) and RBS (BBa_B0034) and FXR-CadC (BBa_K4164026) by homologous recombination.


Source

This sequence is composed of BBa_J23105, BBa_B0034 and BBa_K4164027.

References